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Yadav, Sanjay Kumar
- Combined Mutagenic Improvement of Bacillus licheniformis SK7 for Cost-Effective Protease Production
Authors
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, M.D. University, Rohtak (Haryana), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 91-94Abstract
The strain improvement of developed Bacillus licheniformis SK7 was achieved with the combination of physical and chemical agents i.e., UV + NTG + EMS by gradually mutation and positive selection. In case of UV light 99 per cent killing for successful selection of mutants was 6 min and 10 cm distance from source, while in case of EMS and NTG the concentration found effective was 200 μM, 30 μM, respective for 15 min. The mutant Bacillus licheniformis SK7 SN 43 was successfully developed and found stable having higher production of protease (662 U/ml) under optimized medium and physical conditions of fermentation than the wild type Bacillus licheniformis SK7 (472 U/ml) under the same conditions of fermentation.
Keywords
Mutagenesis, NTG, UV Light, Strain Improvement, Protease.- A Review-Living with Butterfly Skin Disease
Authors
1 Saroj Institute of Technology & Management, Ahimamau P.O. Arjunganj, Sultanpur Road, Lucknow-226002, IN
Source
Asian Journal of Pharmaceutical Research, Vol 3, No 3 (2013), Pagination: 144-150Abstract
Epidermolysis bullosa (EB) is an inherited connective tissue disease causing blisters in the skin and mucosal membranes, with an incidence of 1/50,000. Its severity ranges from mild to lethal. The skin is extremely fragile: minor mechanical friction or trauma will separate the layers of the skin and form blisters. People with this condition have an increased risk of cancers of the skin, and many will eventually be diagnosed with it as a complication of the chronic damage done to the skin. The skin has three layers; the outermost layer is the epidermis, and the middle layer is the dermis. In individuals with healthy skin, there are protein anchors between the layers that prevent them from moving independently from one another (shearing). In people born with EB, these top skin layers lack the protein anchors that hold them together, and any action that creates friction between them (like rubbing or pressure) will create blisters and painful sores. Sufferers of EB have compared the sores with third-degree burns. Current clinical research at the University of Minnesota has included a bone marrow transplant to a 2-year-old child who is one of 2 brothers with EB. Sulforaphane, a compound found in broccoli, was found to reduce blistering in a mouse model to the point where affected pups could not be identified visually, when injected into pregnant mice (5 μmol/day = 0.9 mg) and applied topically to newborns (1 μmol/day = 0.2 mg in jojoba oil).Keywords
Extremely Fragile, Protein Anchors, Broccoli.- Impact of Goods and Service Tax (GST) on Economy of India
Authors
1 National Law Institute University, Bhopal, Kerwa Dam Road, Bhopal, M.P., IN
Source
Indian Journal of Economics and Development, Vol 2, No 5 (2014), Pagination: 1-5Abstract
Objectives: To analyze the impact of GST on the existing indirect tax regime and on the economy of India.
Methods: Doctrinal method was adopted to study the objectives of the research. Researcher has made comparative study of various countries who has adopted GST to replace the existing tax regime and its impact on their economy. Researcher has also critically analyzed the first discussion paper on goods and services tax in India released by empowered committee of state finance ministers in the year 2009.
Findings: GST will play a major role in the growth of the economy of India. After the enforcement of GST in India, GDP will also grow ranging from 0.9% to 1.7%. GST will not only bring reform in indirect tax regime in India but also play a very important role in the growth of trade, commerce and other sectors in India. With the growth of trade, commerce and manufacture sectors in India will be very helpful in generating the new employments. GST will be very helpful in bringing down the inflation and beneficial to the consumer also. In place of dual GST single national tax is require to introduce in order to bring the nation in single tax regime. In case of high rate of GST, there will be changes occur in the existing indirect tax regime. High rate of GST will be equivalent to the cumulative of existing indirect taxes on the products.
Application/Improvements: GST will bring reforms in the indirect tax system and helpful in the growth and development of economy in India.
Keywords
GST, Inflation, Employment, Consumer, Economy, GDP.References
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